PHYSICOCHEMICAL CHARACTERIZATION AND QUANTIFICATION OF TOTAL ANTHRAQUINONES OF VISMIA GUIANENSIS (AUBL.) CHOISY
AbstractVismia guianensis is a plant species of high occurrence in the Amazon and northeastern Brazil, which has been studied lately, because of its antimicrobial and anticancer properties.In order to make an herbal medicine, National Agency for Sanitary Surveillance of Brazil, recommends the production of a drug and plant extracts analysis report indicating: method, specifications and results obtained. The drug was characterized by particle size, amount of water and the total ash content; and plant extract through its density, pH and dry residue. The quantification of total anthraquinones and method validation was done by UV-VIS spectroscopy. The drug in this study produced a coarse powder whose determination of water is within the acceptable limits and the total ash content was 1.64%. The extractive solution showed a low pH, density of 0.89 mg/mL and 9.5% on yields from solids. HPLC found that the EtOAc fraction of the plant extract is consisted of phenolic compounds and anthraquinones. And the method of quantification of total anthraquinones, using emodin as external standard, was validated by UV-VIS spectroscopy at λ 290 nm, determining that in 1 g of extractive solution there are 42.85 mg of total anthraquinones. The results obtained served as reference for the quality control of drug and plant derivative of this species; and the validation of the quantification method showed a high performance.
Article Information
23
2064-2070
738KB
1143
English
IJPSR
Sarah Regina Pereira Camelo , Russany Silva da Costa , Flavio Vasconcelos , Francisco Martins Teixeira , Roseane Maria Ribeiro-Costa , Wagner Luiz Ramos Barbosa and Jose Otavio Silva Junior Race*
Laboratory of Pharmaceutical Research and Development, College of Pharmacy, Institute of Health Sciences, Federal University of Pará, University City “José da Silveira Netto”, CEP 66075-110, Belém, Pará, Brazil
27 February, 2012
24 April, 2012
26 June, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(7).2064-70
01 July, 2012
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