PREPARATION AND CHARACTERIZATION OF NANO-BIO HYBRID BASED MAGNETO LIPOSOME

The biological synthesis of magnetite nano-bio hybrid using plant extracts plays an important role in the field of nanotechnology. The present study demonstrates bio-reductive synthesis of protein coated magnetite (Fe₃O₄) nano-bio hybrid using leaf extracts of Datura inoxia plants and its encapsulation into liposome. Liposome is vesicles of varying size consisting of a spherical lipid bilayer and an aqueous inner compartment that are generated in vitro. Magnet-driven liposome used for the targeted delivery of drugs to organs and tissues. These liposome preparations contain encapsulated drug components and finely dispersed magnetic particles inside the liposome. Liposome is useful in terms of biocompatibility, biodegradability, low toxicity and can control bio distribution by changing the size, lipid composition, and physical characteristics. Furthermore, liposome can entrap both hydrophobic and hydrophilic drugs and are able to continuously release the entrapped substrate, thus being useful drug carriers. The nano bio hybrid based magneto liposome has been prepared by reversed phase evaporation (REV) method using oleic and linoleic acid which has been shown to be driven under magnetic field.  The magneto liposome was characterized by chemically using potassium thiocyanate (KSCN) and confirmed by UV-Vis spectrophotometer Cary 60, Agilent. Magnetic liposome has an advantage over free magnetic nanocores as various functional groups can be attached to the surface of liposome for ligand-specific targeting. Such aspects of magnetically-driven liposome preparations can be used for the targeted delivery of drugs to organs and tissues.