DEVELOPMENT AND VALIDATION OF RATIO DERIVATIVE SPECTROPHOTOMETRIC FOR SIMULTANEOUS ESTIMATION OF METOLAZONE AND SPIRONOLACTONE IN PHARMACEUTICAL DOSAGE FORM
AbstractIn the present work for the simultaneous estimation of Metolazone (METO) and Spironolactone (SPI) Ratio Derivative Spectrophotometric method have been developed. In this method the overlapping spectra of Metolazone and Spironolactone were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. Methanol:water (1:4) was used as solvent for the method. Amplitude at 241.0nm and 263.0 nm were selected in the ratio derivative spectra to determine METO and SPI, respectively. Beer’s law was obeyed in the concentration ranges of 1-5μg/mL and 5-25 μg/ mL METO and SPI, respectively in the method. The % assay for commercial formulation was found to be in the range 98.00% – 102.00 % for METO and 99.00% – 102.60 % for SPI by the proposed method. Recovery was found in the range of 99.74 – 100.76 % for METO and SPI in the Formulations. The results of analysis have been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods which were carried out by following ICH guidelines
Article Information
68
3999-4003
585KB
1079
English
Ijpsr
Arati Chaudhary*, K.R. Vadalia and Punam Thummer
Department of Quality Assurance, Shree H. N. Shukla Institute of Pharmaceutical Education and Research, Rajkot-360002, Gujarat, India
artichaudhary1912@gmail.com
10 June, 2012
20 July, 2012
26 September, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(10).3999-03
01 October, 2012
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