A STUDY ON PHYTOCHEMICAL INVESTIGATION OF DRYNARIA QUERCIFOLIA LINN RHIZOME

In the present research investigation we extracted the powdered rhizome of Drynaria quercifolia linn by Soxhletion method using different solvents. Then extracts were subjected to preliminary phyto-chemical investigation. The proximate analysis was carried out for the plant rhizome powder. The total ash value was 9.93%, Acid insoluble ash value was 4.49%, and Water-soluble ash value was 6.96% and extractive values of alcohol and water was found to be 9.87% and 13.94%. The materials were subjected to successive extraction with solvents. The solvents used were petroleum ether, chloroform, methanol and water in the ascending order of polarity. Pet ether extract was Light brown color, highly viscous and sticky and the yield was 3.12%, chloroform extract was brownish black color, viscous and sticky and the yield was 5.72%, methanol extract was brown color and sticky and yield was 19.67%, and the water extract was brown and sticky and yield was 16.33%. All the extract was subjected for qualitative chemical evaluation to detect the phyto-constituents present in them. Pet ether extract revealed the presence of phytosterols and fixed oils and fats. Chloroform extract revealed the presence of sterols, methanolic extract shows the presence of alkaloids, sterols and tannins and the water extract has shown the presence of tannins, proteins, amino acids, carbohydrates, gums and mucilages. To identify the constituents present in different extracts, the TLC was performed.