IN VITRO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF BARK OF CORDIA DICHOTOMA FORST

In this study, the methanol extract of bark of Cordia dichotoma Forst., belonging to the family Boraginaceae was prepared by successive extraction procedure ( petroleum ether, ethyl acetate, methanol) in soxhlet apparatus and subsequently, the extract was evaluated for its phytoconstituents and pharmacological activity. Comparatively, higher yield (7.11%) of methanol extract was obtained. Phytochemical tests revealed the presence of alkaloid, glycosides, proteins, amino acids, phenolic compounds, flavonoids, tannins and saponins in the extract. The methanol extract was evaluated for its in vitro antioxidant property by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical assay; sodium nitroprusside generated nitric oxide (NO), hydrogen peroxide (H₂O₂₎.Total phenolic content and total flavonoid contents were also carried out. The extract showed IC₅₀ values of 62.46 µg/mL, 48.75µg/mL, 63.5 µg/mL for DPPH free radical, total phenolic content and flavonoid content, respectively. The current study is carried out to investigate the free radical scavenging potential of methanolic extract of bark of Cordia dichotoma using in-vitro models. These models demonstrate antioxidant activity in a concentration dependent manner.