WOUND HEALING ACTIVITY OF LEAF OF NYCTANTHES ARBOR-TRISITIS (LINN.)

Context: The leaves of Nyctanthes arbor-trisitis (Linn.) are used in Traditional System of Medicine for the treatment of stomachic, carminative, intestinal astringent, expectorant, biliousness piles, hair tonic and wound healing. Objective: To evaluate wound healing activity of Nyctanthes arbor-trisitis (Linn.)

Material & Methods: Extracts from the dried leaves of Nyctanthes arbor-trisitis (Linn.) were prepared using methanol as solvent in order to investigate the wound healing activity in vivo. Circular excision and linear incision wounds were created on rats. Three groups of rats were prepared viz. control, standard and treated with extract. The methanol extract of Nyctanthes arbor-trisitis (Linn.) was tested for wound healing activity.

Results & Discussion: The wounds were monitored and the area of wound was measured on 4, 8, 12, 16 post-wounding days and the mean % wound closure were reported. Epithelization period was calculated as the number of days required for falling of the dead tissue remnants without any residual raw wound. Wound healing rate was measured using formula (Muthusamy et al., 2008). Significant wound healing activity was observed for the ointment prepared with methanol extract at 2% (w/w) concentration and with aqueous extract at 2% (w/w) concentration.

Conclusion: The experimental data revealed that the methanolic extract of Nyctanthes arbor-trisitis (Linn.) leaves displayed remarkable wound healing activity.