SIMULTANEOUS ESTIMATION OF CETRIZINE DIHYDROCHLORIDE AND AMBROXOL HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION BY A NOVEL HPLC METHOD
AbstractA rapid and sensitive high performance liquid chromatography method for determination of cetirizine dihydrochloride and ambroxol hydrochloride has been developed. The chromatography system used a reversed phase C-8 column with UV- Vis detection at 230 nm. Mobile phase consisted of acetonitrile – 0.1% triethlamine (50:50 v/v) (pH adjusted to 4.0 using 10% ortho phosphoric acid) at a flow rate of 1.5 ml/min using propranolol as internal standard (I.S.). The calibration curve was linear in the concentration range of 2-20 µg/ml for cetirize dihydrochloride, 24-240µg/ml for ambroxol hydrochloride. The lower limit of detection was found to be 0.06 µg and 0.02µg, for cetirizine dihydochloride and ambroxol hydrochloride respectively.
Article Information
16
3149-3151
390
1060
English
Ijpsr
Pragi Arora*, Varun Arora and Sandeep Jain
Associate Professor, R.K.S.D. College of Pharmacy, Kaithal- 136027, Haryana, India
08 August, 2011
05 November, 2011
02 December, 2011
http://dx.doi.org/10.13040/IJPSR.0975-8232.2(12).3149-51
01 December, 2011
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