INHIBITION OF ANGIOTENSIN CONVERTING ENZYME (ACE) ACTIVITY BY SOME INDONESIA EDIBLE PLANTS
AbstractAntihypertensive properties of plant can be evaluated by in vitro method on inhibition of Angiotensin Converting Enzyme (ACE) activity. In this research, we investigate the inhibitory effect of several common edible plants on blocking ACE activity. ACE activity was evaluated by using N-hippuryl-L-histidyl-L-leucine (HHL) as substrate and the inhibitory effect of extracts were determined based on the level of hippuric acid by measuring its absorbance using spectrophotometer. Gelatin-salt block test for detection of tannins was carried out prior to the enzymatic assay. Among the extracts tested, Peperomia pellucida L. showed strong inhibitory activity with IC50 value of 7.17μg/mL, followed by Nasturtium officinale and Sesamum indicum L. with IC50 values of 15.44 and 30.16μg/mL, respectively. Furthermore, Peperomia pellucida was the most potent inhibitor of ACE activity in vitro with IC50 value of 7.17 µg/ml, which was comparable to that of captopril (13.68 µg/ml), a potent ACE inhibitor. This study shows that these plants may develop as antihypertensive agent
Article Information
13
1054-1059
424
2241
English
Ijpsr
F. C. Saputri *, A. Mun’im , D. Lukmanto, S. N. Aisyah and J. S. Rinandy
Pharmacology Laboratory, Faculty of Pharmacy, University of Indonesia, Kampus UI Depok-West Java , Indonesia
fadlina_chs@yahoo.com
22 July, 2014
29 September, 2014
01 December, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.6(3).1054-59
01 March, 2015
Download