EVALUATION OF ANTIOXIDANT ACTIVITIES OF ALGAE AND EFFECT OF SOLVENT EXTRACTION SYSTEM
AbstractThe present communication is mainly focused to study the antioxidant activities of the four different extracts (benzene, chloroform, acetone and methanol) of five algae including Aphanothece pallida, Anabena variabilis, Aphanothece saxicola, Lyngbya major, Nitella flagelliformis. The total phenolic content varied from 2.99±0.23 to 25.38±0.22 mg/g, 4.20±0.135 to 14.10±0.13 mg/g, 3.07±0.22 to 27.03±0.11 mg/g and 2.34±0.03 to 4.87±0.14 mg/g dry material in the benzene, chloroform, acetone and methanol extracts of the algae respectively. The flavonoid content and flavonol content were found to be highest in the chloroform extract of Nitella flagelliformis and the least amount of flavonoids present in the acetone extract of Lyngbya major (4.88 ± 0.14 mg/g). The 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging effect of the extracts were determined spectrophotometrically. The highest radical scavenging was observed in the acetone extract of Nitella flagelliformis with IC50 = 0.15±0.01 mg dry material. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by the acetone extract of Nitella flagelliformis. The reducing power of the extracts of the plants were also evaluated as mg AAE (ascorbic acid equivalent)/g dry material. The chloroform extract of Nitella flagelliformis showed the highest reducing power 231.08±2.01 mg AAE/g dry material. The results indicate that these algae can be utilized as natural antioxidant in order to prevent free radical toxicity
Article Information
44
1273-1278
335
1123
English
Ijpsr
Tapan Seal *, Nilu Halder, Kausik Chaudhuri and Sankar Narayan Sinha
Plant Chemistry Department , Botanical Survey of India, A. J. C. Bose Indian Botanic Garden, Shibpur, Howrah, India
kaktapan65@yahoo.co.in
29 July, 2014
29 September, 2014
01 December, 2014
DOI: 10.13040/IJPSR.0975-8232.6(3).1273-78
01 March, 2015