HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY OF METHANOLIC BARK EXTRACT FROM ALBIZIA LEBBECK LINN.
AbstractIn the present study, high performance thin layer chromatographic method was developed and quantification of Diosgenin from Albizia lebbeck. TLC auminium precoated with silica gel were used as the stationary phase in a twin see through glass chamber saturated with n-butanol: glacial acetic acid: water (4:1:1) mobile phase, performed at room temperature (25ºC ± 2). The Rf value of diosgenin was found to be 0.8. Linearity was found to be in the concentration range 2 to 10 µg/spot. The linearity regression analysis of calibration plots showed good linear relationship between peak area and peak height (r2= 0.996). The result showed that the chromatogram of Diosgenin was performed in 3D display of diosgenin and Overlay of diosgenin and ethanolic bark extract of Albizia lebbeck.
Download