ESTIMATION OF PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF CALOTROPIS GIGANTEA EXTRACT: WOUND HEALING ACTIVITY AND ITS BIOMEDICAL APPLICATION

Wound is unpreventable consequences of life, which originates due to physical damage, chemical injury and microbial pathogenic infections, which extend to loss of cellular and functional continuity of living tissue. Calotropis gigantea is a weed plant it used as a wound healing agent. Calotropis gigantea is a waste land weed. Antioxidants represent a significant function to assist human, against infections and chronic diseases. The exhibit investigation has been contained to assess the phytochemicals and invitro antioxidant activity of Calotropis gigantea. Phytochemicals were analyzed qualitatively and the result sustained the presence of alkaloids, phenols, saponin, and steroids. The invitro antioxidant activity of root was investigated by DPPH (1, 1-Diphenyl-2-picrylhydrazyl) and Super oxide free radical scavenging activity method. In both methods, plant extract possess high antioxidant activity when equated with standard ascorbic acid due to presence of high capacity of various phytochemicals. The pro-wound healing activity of the Calotropis gigantea extract may be due to its high content of glycosides, flavonoids, phenolic compounds, and triterpenoids with antimicrobial and antioxidant properties. From the results obtained, it may be concluded that Calotropis gigantea extract has the potential to be developed into new therapeutic agent for wound healing.