METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF TINIDAZOLE BY REVERSE PHASE HPLC TECHNIQUE
AbstractThe aim of the current study is to develop a simple, specific, rapid and precise quantification technique for the estimation of tinidazole from tablet dosage form. Successful separation of the drug was carried out on a C18 column (particle size 5 µm, 250 mm length × 4 mm i.d.) using a mobile phase consisting of a 5.3 mM phosphate buffer solution and acetonitrile in the ratio of 60:40 (v/v). The detection wavelength is 318 nm. The method has been validated as per ICH (Q2) guidelines on the basis of accuracy, precession, linearity, sensitivity and robustness. The method is found to be linear with limit of detection and limit of quantitation 0.25μg/ml and 0.76μg/ml respectively. The average elution time is only 5.0 minutes with the analyte elution taking place at about 3.0 minutes making the method rapid and cost effective for routine analysis
Article Information
56
3594-98
361
1345
English
Ijpsr
Amit Kumar De , Ashok Kumar Bera and Biswajit Pal*
Department of Chemistry , St. Paul’s C. M. College, Kolkata, West Bengal, India
palbiswajit@yahoo.com
27 January, 2015
19 March, 2015
11 May, 2015
10.13040/IJPSR.0975-8232.6(8).3594-98
01 August, 2015
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