BACOPAMONNIERA, A NOOTROPIC HERB ALLEVIATES ABNORMAL MOLECULAR AND IMMUNOHISTOCHEMICAL CHANGES EVOKED BY CHRONIC EXPOSURE OF METHYLMERCURY IN RAT CEREBELLUM
AbstractMethylmercury (MeHg), a highly toxic environmental pollutant, induces oxidative stress and dysfunction in many cell types. Bacopa monniera is a perennial herb, and is used as a nerve tonic in ayurveda, a traditional medicinal system in India. This study was aimed to evaluate the effect of Bacopa monniera extract (BME) on MeHg-induced toxicity in rat cerebellum. Male Wistar rats were administered with MeHg orally at a dose of 5 mg/kg b.w. for 21 days. Experimentalrats were given MeHg and also administered with BME(40 mg/kg, orally) 1 h prior to the administration of MeHg for 21 days. After treatment MeHg induction caused increased level of Scgb3a1mRNA whereas the level was reduced by BME pretreatment. GPx4 expression was down regulated and HSP70 expression was up regulated in MeHg intoxicated group. Immunohistochemical changes were observed in TRxR1 and GFAP protein in MeHg induced group. BME pretreatment altered the expression of proteins and Immunohistochemical changes to near normal. These findings strongly implicate that BM has potential to protect brain from oxidative damage resulting from MeHg-induced neurotoxicity in rat.
Article Information
61
3626-33
615
912
English
Ijpsr
Johnson Christinal and Thangarajan Sumathi*
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai , Tamil Nadu, India
drsumathi.bioscience@gmail.com
19 December, 2014
19 December, 2014
05 May, 2015
10.13040/IJPSR.0975-8232.6(8).3626-33
01 August, 2015
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