VIABILITY OF ADIPOSE-DERIVED STEM CELLS (ASCs) ON POROUS CHITOSAN SCAFFOLD
AbstractBackground: Stem cells have great potential in regenerative medicine due to their multipotentiality. Treatments involving cell sheets for tissue reconstruction require a large number of cells to repair the tissue damage, and the cells are fragile. Combined with a porous chitosan scaffold (PCS), adipose-derived stem cells (ASCs), which are ubiquitous, can be delivered to a target tissue in vivo. This approach provides mechanical support to the skin until the normal tissue is regenerated. In the present study, ASCs were characterized and tested for their viability on a PCS in vitro prior to in vivo application. Results: The ASCs expressed CD29, CD73, CD90 and CD105 but did not express CD34, as demonstrated by immunocytochemistry. Most of the ASCs were found to be viable on the PCS at 24 and 72 hours of culture. These results suggest that ASCs seeded on a PCS have high viability, allowing ASCs to be enriched for therapeutic applications. Conclusions: This qualitative study has proven ASCs were able to survive on PCS as indicated by the number of live cells exceeded the number of dead cells as demonstrated by live/dead assay
Article Information
15
3781-87
684
1062
English
Ijpsr
M. Mohamed , S. Gomathysankar , AZ Mat Saad , Kartini Noorsal and AS Halim *
School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
ashalim@usm.my
09 February, 2015
18 April, 2015
29 May, 2015
10.13040/IJPSR.0975-8232.6(9).3781-87
01 September, 2015
Download