ANTI-INFLUENZA POTENTIAL OF ALKALOIDAL MOLECULES OF JATROPHA CURCAS LEAVES
AbstractBackground: The Influenza viruses are major etiologic agents of human respiratory infections, and inflict sizable health and economic burden. Methods: The present study reports the in-vitro antiviral effect of isolated alkaloidal compound of Jatropha curcas leaves against Influenza virus by Hemagglutination (HA) reduction assay in two different layouts of simultaneous and post-treatment assay. The alkaloidal compound was used for anti-influenza activity in the non-cytotoxic range. The separated compound (Rf 0.5) was eluted, crystallised and identified by using NMR and FTIR analysis and co-TLC with standard compound of Tetra Methyl Pyrazine(TMP). Results: The Thin Layer Chromatographic analysis showed that ethyl acetate: dichloromethane (1:1) was the best solvent for alkaloidal separation. The identified Tetra-Methyl Pyrazine showed 100% reduction in HA in the post-treatment assays at the concentration of 10mg/ml and 5mg/ml. In simultaneous assay, HA was reduced to 71% and 42% at concentration of 10mg/ml and 5mg/ml respectively. Conclusions: These results suggest that TMP has strong anti-Influenza virus activity that can inhibit viral attachment and/or viral replication and may be used as viral prophylaxis
Article Information
17
4705-11
385
1213
English
IJPSR
Rahul Chavan*, Pratibha Chaturvedi and Abhay Chowdhary
Department of Virology & Immunology, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, Maharashtra, India
raulchavan@gmail.com
25 April, 2015
02 June, 2015
10 September, 2015
10.13040/IJPSR.0975-8232.6(11).4705-11
01 November, 2015
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