EFFICACY OF NON POLAR EXTRACT (NPE) OF ALOE BARBADENSIS MILL. IN POLYCYSTIC OVARIAN SYNDROME (PCOS) RODENT MODEL- AN “IN VIVO” STUDY

Aim of the current study was to evaluate the efficacy of non polar extract (NPE) of Aloe vera gel (AVG) for 60 days (~25μg/daily/orally) in rodent model of polycystic ovarian syndrome (PCOS). After treatment, steroid hormones and insulin levels were estimated by ELISA, serum lipid profile along with ovarian steroidal enzyme activity. Also, transcript as well as protein level of key receptors and steroidogenic enzymes were evaluated. Parallely, pairs of ovaries were excised and stained with Hematoxylin and Eosin (HE) to monitor the structural changes from group of animals. During course of study, stability of non polar extract (NPE) of AVG was evaluated by High Performance Thin Layer Chromatography (HPTLC) during study (15, 30, 45, 60 days). PCOS rats after treatment with NPE demonstrated a decrease in serum testosterone and insulin level with improved estradiol and progesterone levels. Also, decrease in transcripts level of steroid receptors while increased aromatase expression were observed after NPE treatment. These genes expression study was correlated with relative changes were observed with protein expressions. Non polar phytosterol rich fraction treated PCOS rats improved metabolic phenotype as well as ovarian function. These modulations ameliorate the PCO condition suggesting that these could possible novel targets in management of PCOS.