CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF BIOGENIC SILVER NANO-PARTICLES USING LEAF EXTRACT OF THUNBERGIA ALATA BOJER EX SIMS

The multidrug-resistant strains are a major problem in the control of infections in hospitals. The smaller size of nanoparticles is gaining importance in research for the treatment of various diseases. The main objective of this study is synthesis of silver nanoparticle in eco-friendly manner without using any hazardous chemicals. Stable and spherical shaped nanoparticles Synthesized by using aqueous leaf extract of T. alata. This method offers a viable and an eco-friendly way for fabrication of benign nanoparticles as it is a simple and carried out at room temperature without any huge inputs in terms of energy and waste. It is advantageous over the microbial synthesis as it is carried out using in aqueous solutions at ambient temperature, without any toxic chemicals in lesser time and could be exploited for developing cost effective biosynthesis of Ag nanoparticles at a large scale. One more aim of this study is that analysis of antimicrobial activity of biogenic nanoparticles against disease causing human pathogens. Characterizations of nanoparticles were done by UV-Vis spectroscopy, FTIR, AFM, HR-TEM, XRD and with EDS instruments. Antimicrobial test was done by agar well diffusion method. The nanoparticles appeared to be spherical in shape and the size of the particles varied from 10 to 50 nm. T. alata leaf extract mediated silver nanoparticles have great promise as antimicrobial agent against human pathogenic multidrug resistant microbes.