BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NITROFURANTOIN IN HUMAN PLASMA BY LC -MS / MS
AbstractA simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of nitrofurantoin in the Human Plasma. The Mass transition of nitrofurantoin and losartan (Internal standard) were M/z 237.1/151.9 and M/Z 421.300/ 179 in ESI Negative ionization. Linearity was observed between the nitrofurantoin concentration and the peak area ratio from 10.451 to 1033.897 ng/mL with R2 value of 0.99. Plasma samples containing nitrofurantoin were extracted with Acetonitrile: 5mM Ammonium Acetate (pH 3.8) (80:20). The observed recovery of nitrofurantoin was 90%. The intra-day and inter-day accuracy was performed. Stability parameter was performed. The method will be used in the determination of the pharmacokinetic parameters of nitrofurantoin.
Article Information
16
4186-4194
634
1378
English
IJPSR
M. S. Kumar* and P. Shanmugapandiyan
V-102, Hivision Residency, Backside Siddh Convention, Kompally, Hyderabad, Telangana, India.
sathishmeruva85@gmail.com
25 February, 2017
09 May, 2017
27 May, 2017
10.13040/IJPSR.0975-8232.8(10).4186-94
01 October, 2017
Download