ANTIDIABETIC ACTIVITY OF METHANOLIC LEAVES EXTRACTS OF TRANSFORMED SOYBEAN IN 3T3-L1 PRE-ADIPOCYTES CELL LINE

The present study was initiated to quantify the Isoflavones content that accumulated in soybean transgenics with RnPPARγ gene by using high performance liquid chromatography. The effects of methanolic leaves extract of transformed soybean in 3T3-L₁ adipocyte cell line. The RnPPARγ transgenic soybean variety JS335 was developed and transgenics were confirmed by PCR and Southern blot techniques. The quantification of isoflavones compounds by HPLC showed that methanolic leaves extracts of transformed soybean contained Genistein (1.67µg/g extract), Daidzein (2.76µg/g extract), Rutin (32.06µg/g extract) and Quercetin (48.40µg/g extract) and methanolic leaves extracts of non-transformed soybean contained Genistein (1.23µg/g extract), Daidzein (2.33µg/g extract), Rutin (26.16µg/g extract) and Quercetin (43.03µg/g extract) by comparing the chromatogram of the reference standards. In the present study revealed that secondary metabolite content was higher in the transformed plant than non-transformed plants. After treatment of transformed methanolic leaves, extracts on 3T3-L₁ mature adipocytes significantly ameliorated lipid droplet accumulation and droplet reduction in adipocytes might be the result of lipid metabolism by lipolysis.