ANTIPROLIFERATIVE ACTIVITY OF RUTIN ON HELA CELL LINE INDUCED CERVICAL CANCER IN RATS

In present study antiproliferative activity of Rutin was evaluated on HeLa cell line induced cervical cancer in rats. For this study, 30 rats were divided into 5 groups and each group containing 6 rats each. Group I- normal saline treatment for 45 days, Group II- cancer cells (1×10⁶ cells in 0.1ml/rat), Group III– 5-Fluorouracil (20mg/kg + 1×10⁶ cells in 0.1ml/rat), Group IV- Rutin (50mg/kg + 1×10⁶ cells in 0.1ml/rat), Group V- Rutin (70mg/kg + 1×10⁶ cells in 0.1ml/rat). After 24 h of tumour inoculation intraperitoneally, Rutin was administered daily for 45 days. After administration of last dose followed by 18 hrs fasting, rats were sacrificed for observation of antiproliferative activity. The change in body weight, body circumference of tumour bearing hosts and simultaneous alterations in haematological profile, serum (Triglycerides, Total protein, Total cholesterol, GGT, ALP and glucose) and liver biochemical parameters (lipid peroxidation, GSH and antioxidant enzymes-CAT, GPx) were estimated. The changes in tissue enzymes-Glucose-6 phosphate dehydrogenase, Hexokinase, Succinate dehydrogenase and CytochromeP450 levels were also estimated. Rutin maintained the body circumference and body weight of proliferation bearing rat. Haematological profile reverted towards normal levels in Rutin treated rat. Treatment with Rutin restored serum biochemical parameters towards normal levels and decreased levels of lipid peroxidation and increased levels of reduced glutathione and other antioxidant enzymes. The Rutin treatment restored Glucose-6 phosphate dehydrogenase, Hexokinase, Succinate dehydrogenase and CytochromeP450 levels in proliferation induced rat. Rutin exhibited antiproliferative effect by modulating haematological parameters, lipid peroxidation and augmenting antioxidant defense system in proliferation bearing rat.