ANTITUMOUR AND ANTIOXIDANT ACTIVITY OF WRIGHTIA TINCTORIA (ROXB.) R. BR. LEAF OIL
AbstractThe present study is based on the evaluation of preliminary phytochemical screening, antitumor, cytotoxic and antioxidant activities of leaf oil of Wrightia tinctoria (Oil B). The oil B from Wrightia tinctoria leaves was subjected to phytochemical screening tests by using standard procedures. The free radical scavenging activity of both the extracts was measured by using the 1, 1- diphenyl-2-picrylhydrazyl (DPPH) assay. The dose dependent increase was observed in DPPH activity of leaf oil B (0.125, 0.25, 0.50, 1 μg/ml). Oil B was investigated for its short term cytotoxicity on DLA cells by tryphan blue dye exclusion method. In vivo antitumor activity was studied on DLA tumor bearing mice. Activity was assessed by monitoring the mean survival time and solid tumor volume. Wrightia tinctoria leaf oil was proved to be an effective anticancer and antioxidant agent. These results suggest that the oil B has potential of antioxidant and antitumor activity that support the ethno pharmacological uses of this plant. The remarkable activity showed by the plant could be attributed to the synergic effect of the active compounds present in it.
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