DEVELOPMENT OF QUALITY CONTROL PARAMETERS FOR THE STANDARDIZATION OF AEGLE MARMELOS (ROXB.) SEED

The present investigation was undertaken to determine the requisite pharmacognostic standards for evaluating the plant material. The macroscopic, microscopic, powder study, physicochemical, qualitative phytochemical and florescence analysis of the seed were carried out. The microscopic study showed abundant large parenchymatous cells with aleurone grains, calcium oxalate crystals, lignified trichomes, endocarp and testa throughout the section. The powder microscopy showed the presence of lignified trichomes, rossete crystals of calcium oxalates, oil granules, etc. The physicochemical properties such as total ash, water soluble ash, acid insoluble ash and sulphated ash were 5.75%, 5.16%, 0.16% and 6.66% respectively. The aqueous soluble and ethyl acetate soluble extractive values were 34.07% and 16.66% respectively. The qualitative phytochemical analysis showed the presence of maximum amount of flavonoids followed by triterpenes, steroids and alkaloids respectively. The seed powder showed characteristic flourescence with various chemical reagents. The data generated in the present work could be used as reference for the standardization and quality control of A. marmelos seed. It will help in identifying and preventing intentional or unintentional adulteration of this plant material.