ASSESSMENT OF ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF ANNONA RETICULATA (L.)

Objective: The present study has been carried out to evaluate the antioxidant property of different extracts obtained from successive extraction of Annona reticulata L. leave with solvents of increasing polarity. Effort also has been made to estimate the flavonoid and phenolics content of the extracts. Method: The antioxidant activity has been studied in-vitro by using DPPH, hydrogen peroxide and nitric oxide-scavenging assay. Results: DPPH, H₂O₂ and Nitric-oxide scavenging effect of Annona reticulata L.  leaf extracts  were found  to  be  dose  dependant  with  maximum  inhibition   at  highest concentration. Methanolic extract of the leaves of Annona reticulata Linn. is found to have most potent anti-oxidant activity in DPPH, H₂O₂ and nitric-oxide Radical scavenging methods. IC₅₀ value DPPH, H₂O₂ and Nitric-oxide inhibition of methanolic extract at highest concentration (400µg/ml) are 62.58 ± 1.15, 68.27 ± 1.05 and 64.01 ± 1.02. Conclusion: The results suggest that all the tested extracts are having antioxidant property, but the methanol is having significantly higher flavonoid and phenol content. Due to presence of higher flavonoid and phenol content in methanol, it may be considered as the fraction with better pharmacological property in comparison to other tested extracts.