THE SYNERGESTIC EFFECTS OF EDTA / ANTIBACTERIAL COMBINATIONS IN KLEBSIELLA PNEUMONAE INDUCED PNEUMONIA RAT MODEL

The study was conducted to evaluate the synergistic effect of EDTA with antibacterial (Ceftriaxone + Sulbactum) as a combinational therapy against antioxidant enzyme activity, lipid peroxidation level, some hematological and biochemical parameters in Klebsiella pneumoniae induced nosocomial pneumonia followed by histopathology of lungs tissue in experimental exposed Sprague Dayle (S.D) rats. Total 24 rats were selected and divided into 4 groups of 06 rats each for in-vivo and in-vitro EDTA / antibacterial therapy. Group I Control normal saline treated group; group II was infected via K. pneumoniae bacterial strain. Group III and IV were infected plus treated group with antibacterial and EDTA / Antibacterial drugs. The results indicated that a significant (p<0.005) decrease in enzymes activities (Superoxide dismutase, catalase and ascorbic acid) along with increased lipid peroxidation level as well as cytokine parameters (tumour necrosis factor-α, interlukin-6) and biochemical parameters in  plasma of infected group as compared to control group. These activities were increased along with decreased in lipid peroxidation level and biochemical parameters in both treated group as compared to infected group after seven days treatment with antibacterial and EDTA/Antibacterial drugs. When antibacterial treated group was compared with EDTA/Antibacterial treated group, all above parameters were improved in the plasma of EDTA / Antibacterial treated group. These findings concluded that EDTA / Antibacterial has better synergistic efficacy effect than antibacterial alone in Klebsiella pneumoniae induced nosocomial pneumonia infection.