IN-VITRO ANTI-OBESITY EFFICACY OF SELECTED PLANTS IN 3T3-L1 CELL LINE

Background: Obesity is a confronting global issue with excess accumulation of body fat to an extent that decreases life expectancy. It is multifactorial in origin; genetic, environmental and pathophysiological. It leads to metabolic syndrome characterized by type 2 diabetes, cardiovascular diseases, fatty liver, hyperlipidemia etc. Objective: In this study, we have screened hydroalcoholic extracts of 7 plants for anti-obesity efficacy using inhibitory effect on lipid and carbohydrate metabolizing enzymes as indicators and further screening of these plants in 3T3 L1 cell line for inhibition of adipogenesis. Results: Among the 7 plants screened, Cyperus rotundus and Citrus limon showed highest inhibitory activity compared to other plants. These plants also showed antioxidant activity. Furthermore, these plants were investigated for their inhibition of adipogenesis and promotion of lipolysis in 3T3-L1cells. Cyperus rotundus and Citrus limon at 125 µg/mL showed inhibition of triglyceride accumulation and promoted lipolysis. Conclusion: Over all preliminary in-vitro screening study reveals Cyperus rotundus and Citrus limon showed potent anti obesity efficacy thus these plants may act as alternative herbal approach for treating obesity.