BIOCHEMICAL STUDIES ON THE EFFECT OF ETHANOLIC EXTRACTS OF TRICHOSANTHES DIOICA AND CLITORIA TERNATEA IN STREPTOZOTOCIN INDUCED DIABETIC MALE WISTAR RATS

To investigate the effect of ethanolic extracts of leaf and fruit of Trichosanthe dioica (T. dioica) and leaf of Clitoria ternatea (C. ternatea) on the biochemical parameters in streptozotocin (STZ) induced diabetic Wistar albino rats. Healthy male adult Albino rats of Wistar strain were randomly divided into eleven groups of six rats each were assigned into normal and diabetic groups (Group I to XI). Diabetes was induced in albino rats by administration of STZ (60 mg/kg i.p). Group I and II were kept as normal control and diabetic control. The other diabetic groups from group III to group X were treated with either individual and combined ethanolic extracts of T. dioica and C. ternatea at a dose of 200 and 400 mg/kg of body weight (bw) were administrated at a single dose per day and group XI was treated with glibenclamide at a dose of 600 μg/kg body weight for 28 consecutive days. The effect of ethanolic extracts of leaf and fruit of T. dioica and leaf of C. ternatea on blood glucose, serum insulin, glycosylated hemoglobin (HbA1c), urea, creatinine, SGOT, SGPT, LDH, ALP, total protein, albumin and globulin were evaluated in the diabetic rats. In the acute toxicity study, ethanolic extracts of leaf and fruit of T. dioica and leaf of C. ternatea were non-toxic and safe up to 2000 mg/kg body weight in rats. A significant decrease in blood glucose, HbA1c and other biochemical parameters levels and increase in serum insulin level were observed in diabetic rats treated with ethanolic extracts of T. dioica (leaf and fruit) and C. ternatea (leaf) compared to diabetic control rats. These findings suggest that the combined extract of T. dioica fruit and C. ternatea leaf have potent antidiabetic activity in STZ induced diabetic rats.