IN-VITRO ANALYSIS TO ANALYSE THE DISINTEGERATION PROPERTY OF PRF WHEN TREATED WITH VARIOUS CONCENTRATION OF TRANEXAMIC ACID

Aim: To assess whether Tranexamic acid has any effect in inhibiting degradability of PRF membrane and also to compare the degradability of PRF at different concentrations of Tranexamic acid (200 mg, 150 mg and 50 mg). Material and Methods: The degradability of PRF membrane was compared between various concentrations of Tranexamic acid such as 200 mg, 150 mg, 50 mg against negative control of phosphate buffer solution (PBS). Group 1-PBS (negative control) n = 5, Group 2-PBS containing 200 mg of Tranexamic acid n = 5, Group 3-PBS containing 150 mg of Tranexamic acid n = 5, and Group 4-PBS containing 50 mg of Tranexamic acid n =5. PRF procured from each donor was weighed in a micro weighing machine and they were later dropped in each of the 4 groups of eppendorfs (5 in each group). After 1 week of storing in room temperature, the PRF pieces were retrieved and percentage of remaining PRF was calculated. ANOVA and post hoc tests were used to statistically compared this data between the groups. Results: There was reduction in the weights of the PRF in all the groups at 1 week when compare to baseline. The mean percentage of remaining weight of PRF in groups 1-4 was 35.59 ± 14.23, 85.23 ± 7.23, 87.27 ± 4.28, and 83.65 ± 6.22 respectively. Conclusion: Tranexamic acid at different concentration delayed the degradability of PRF efficiently.