IN-VITRO AND IN-VIVO EFFICACY OF ROOT EXTRACT OF LAWSONIA INERMIS AGAINST INFLAMMATORY PARADIGM

The study was carried out to evaluate anti-inflammatory activity of alcoholic extract of roots of Lawsonia Inermis (LILAC). In-vitro anti-inflammatory activity was evaluated by Cyclooxygenase inhibitory assay wherein the LIALC showed a dose-dependent inhibition of both the enzymes with IC₅₀ values of 58.2 μg/ml for COX-1 and 7.38 μg/ml for COX-2. In RBC membrane stabilization activity, LILAC at the concentration of 100 µg/ml and 200µg/ml exhibited membrane stabilizing activity. Acute oral toxicity of extract was determined by OECD 423 guidelines wherein mortality was not observed when the extract was administered orally up to 2000 mg/kg. Carrageenan-induced paw edema model evaluated in-vivo anti-inflammatory activity; Paw edema was induced by injecting 0.1 ml of 1% w/v carrageenan into the subplantar tissues of the left hind paw of each rat. LILAC at all the three doses, 200, 300 and 400 mg/kg, showed maximum inhibition of 31.8, 40.9 and 50.8% respectively at 180 min. In Cotton pellet-induced granuloma model, Sterile, weighed, cotton pellets (10 ± 1 mg) were implanted subcutaneously in the groin regions of the rats under light anesthesia, the dry weight of cotton pellet was significantly reduced by 36.4, 46.4 and 50.5% at 200, 300 and 400 mg/kg of LILAC respectively. The research findings substantiate the promising anti-inflammatory activity of alcoholic extract of roots of Lawsonia Inermis in all the tested in-vitro and in–vivo models.