EVALUATION OF METHANOLIC EXTRACT OF CHYTRANTHUS MACROBOTRYS SEED (CMS) FOR ANTIMICROBIAL, α-GLUCOSIDASE AND α-AMYLASE INHIBITORY ACTIVITIES

Chytranthus macrobotrys (Sapindaceae) is one of the notable African medicinal plants with traditional history but lack scientific data. This report was aimed to evaluate C. macrobotrys seed (CMS) methanol extract for its phytochemical composition, antimicrobial activity, α-glucosidase, and α-amylase inhibitory activities. Methanolic extract of C. macrobotrys seed was investigated for its chemical composition, antimicrobial activity, α-glucosidase, and α-amylase inhibitory activities. The results revealed the presence of flavonoid (18.61 ± 1.88 mg/100g), phenol (28.71 ± 2.95 mg/100g), tannin (17.29 ± 1.84 mg/100g), terpene (587.07 ± 3.63 mg/100g), sterol (111.54 ± 3.85 mg/100g) and saponin (761.78 ± 1.35 mg/100g). The extract using agar diffusion method showed antimicrobial activity against Staphylococcus aureus, Proteus vulgaris, Pseudomonas aureuginosa, Streptococcus pneumonia, Bacillus cereus, and Micrococcus luteus with a diameter of inhibition zones from 10-20 mm. A weak antimicrobial activity was observed against Escherichia coli and Shigella sp. while no activity was observed against Klebsiella pneumonia and Salmonella typhi. The CMS showed α-glucosidase and α-amylase inhibitory activity at the concentration range of 10-100 µg/ml. The highest % inhibition was observed at 100 µg/ml (41.61 ± 1.85) and (37.90 ± 1.89) respectively. The study nominated CMS as a possible alternative natural remedy in the treatment of microbial infections and diabetes. Therefore, elucidating the active components, mechanistic mode of actions and further subjection of CMS for an in-vivo study are required.