SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF GLIBENCLAMIDE IN PURE FORM AND IN DOSAGE FORM
AbstractTwo simple, sensitive and rapid spectrophotometric methods was developed for estimation of glibenclamide in pure and in pharmaceutical dosage forms. Method-A is a uv spectroscopic method with the absorption maxima at 276nm.Method-B is based on the oxidation/reduction of glibenclamide with iron (III) and chelation of iron with 3-methyl 2-benzothiozoline hydrazone(MBTH) to form green colored complex with absorption maxima at 630nm.Calibration curve was plotted. The assay was validated for the parameters like precision, accuracy, robustness, ruggedness, LOD and LOQ. The proposed methods can be useful for the routine analysis for the determination of glibenclamide in pharmaceutical dosage form.
Article Information
39
4925-4928
367KB
1577
English
IJPSR
N. Umadevi *, CH. Mounika and Dr. I. Sudheer Babu
SIR C. R. Reddy College of Pharmaceutical Sciences, Eluru -534007, Andhra Pradesh, India
umapharma41@gmail.com
08 April, 2014
27 August, 2014
17 October, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(11).4925-28
01 November, 2014
Download