CHARACTERIZATION OF LIGNINOLYTIC ENZYMES AND DECOLOURIZATION OF SELECTED TEXTILE DYES FROM THE BLUSHING BRACKET MUSHROOM, DAEDALEOPSIS CONFRAGOSA

The effect of a wide range of culture conditions on the production of laccase (Lac), Lignin peroxidase (Lip) and Manganese peroxidases (MnP) by Daedaleopsis confragosa was studied. A ligninolytic enzyme-producing bracket mushroom, Daedaleopsis confragosa was isolated from tree trunk from Karwar coastal region, Karnataka, India and subjected to production and dye decolorization study. Ligninolytic enzyme production was done by Submerged Fermentation (SmF). Various process parameters like different pH, temperature, carbon, nitrogen sources, and metal ions were investigated on Laccase (Lac), Lignin peroxidase (LiP) and Manganese peroxidizes (MnP) production in the fermentation process. The study revealed that the Lac, Lip and MnP highest activity was observed at pH 6.0, 5.5, and 7.2 respectively. Lac, Lip, and MnP showed highest activity at temperature 45°C, 40°C and 40°C respectively. Glucose as carbon source and modified PDB (Potato dextrose broth +2% saw dust + 1% yeast extract) as nitrogen source support maximum laccase activity. Starch as carbon source and modified PDB as nitrogen source enhanced the highest activity of Lignin peroxidase and Manganese peroxidases, respectively. Laccase (Lac), Lignin peroxidase (LiP), and Manganese peroxidases (MnP) activity were highest with copper ion supplement. Further textile dye decolorization was performed with Red M8B, Green HE4B, Navy blue HER, Orange HE2R, were decolorized at a range of 80-95%.