EVALUATION OF THROMBOLYTIC ACTIVITY AND BRINE SHRIMP LETHALITY BIOASSAY OF METHANOL EXTRACT OF STEMS OF TINOSPORA CRISPA

Tinospora crispa, a plant belonging to the family of Menispermaceae is a well-known traditional medicinal plant used in Bangladesh. The methanolic extracts of stems of the plantwere evaluated for their thrombolytic and preliminary cytotoxic activities. The thrombolytic activity was evaluated using the in vitro clot lysis model while while the screening of cytotoxic activity was done using brine shrimp lethality bioassay. Different concentrations of stem extract were made in isotonic saline (2.5, 5, 10 and 20 mg/ml) and added to pre-clotted human blood (n=13) and the clot lysis activity was determined following 90 minute incubation at 37º C. The results showed that T. crispa stem extract induced mild to moderate (14.81- 25.73%) and significant (p <0.001) clot lysis activity whereas the standard Streptokinase (30,000 I.U.) evoked a significant (p < 0.001) clot lysis activity (50.1%). In Brine shrimp lethality bioassay, the LC₅₀ values of methanolic crude extract and its pet ether, chloroform and aqueous fractions were found to be   6.43, 0.8. 4.58 and 7.46  mg/ml respectively which were comparable to the standard vincristine sulphate (LC₅₀:  0.107 µg/ml). The results of the study demonstrated that the stem of the plant possesses promising thrombolytic activity in vitro when tested on human blood as well as preliminary cytotoxic activity on Brine shrimp. However, further study is needed to see its potentiality as thrombolytic medicine