CHARACTERIZATION OF BIOLOGICALLY ACTIVE FRACTIONS OF METHANOL EXTRACT OF MATURE LEAVES OF POGOSTEMON BENGHALENSIS (BURM. F.) KUNTZE AND PERILLA FRUTESCENS (L.) BRITTON

The aim of the study was to determine the biologically active fractions of methanol extracts of mature leaves of Pogostemon benghalensis (burm. f.) Kuntze and Perilla frutescens L. Britton. The methanol extract was broadly fractionated sequentially in hexane, chloroform, acetone, ethyl acetate, and methanol. Activity index was recorded higher in methanol broad fractions of both of the plants. Broad fractions of both of the plants recorded a higher Proportion Index against E. coli (1.00). An increasing trend was recorded in the case of total phenol and flavonoid content with an increase in concentration from 1 mg/ml to 5 mg/ml. In case of Pogostemon benghalensis (Burm.f.) Kuntze, antioxidant inhibition of ascorbic acid against DPPH and ABTS are (98.28 ± 1.03% and 96.22 ± 2.01% respectively) which is higher than the crude extract (81.43 ± 0.09% and 72.32 ± 0.01% respectively) at 500 µl of the sample at 1mg/ml of concentration. TLC Spots from hexane and chloroform broad fraction recorded antibacterial inhibition against B. subtilis. R_f values of TLC spots from hexane and chloroform broad fractions of both of the plants were recorded.