AMELIORATIVE POTENTIAL OF AQUEOUS EXTRACT OF MORINGA OLEIFERA LEAF AGAINST IMIDACLOPRID INDUCED HEPATOTOXICITY IN ZEBRA FISH, DANIO RERIO

The current study was aimed to estimate the ameliorative potential of Moringa leaf extract (MLE) against the toxicity of Imidacloprid (IMC) on hepatic biomarkers in zebra fishes, Danio rerio after acute exposure. To analyze liver biomarkers, 20 fishes were randomly selected and divided into four groups. Group- I served as control, Group- II received MLE- 12 ml/l , Group III- treated with 24 h LC₅₀ of IMC (0.423 ml/l), Group- IV received 0.423 ml/l of IMC + 12 ml/l of MLE. The same protocol was implemented by taking 96 hrs LC₅₀ of IMC (0.270 ml/l) and MLE (10 ml/l). At the end of the experiment, liver was dissected out, homogenized, and centrifuged separately to collect supernatant for enzyme analysis. The activity of ALT, ALP, LDH, and GGT were increased in IMC exposed fishes after 24 and 96 h exposure periods, whereas protein contents were decreased. The AST activity was decreased after 24 h and increased after 96 h exposure period. Administration of MLE along with IMC, reversed the impact of IMC. These findings highlight that MLE have great importance in the amelioration of some biochemical disturbances associated with the acute toxicity of IMC.