IN-VITRO ANTIBACTERIAL ACTIVITIES OF EXTRACTS OF YEMENI PLANTS MYRTUS COMMUNIS L. AND FLEMINGIA GRAHAMIANA WIGHT & ARN.

Yemen is located on the southwestern brink of the Arabian Peninsula. The methanolic and aqueous crude extracts of two Yemeni traditional medicinal plants Myrtus communis L. and Flemingia grahamiana Wight & Arn were evaluated for antibacterial activity in-vitro by Well- diffusion method against six bacterial pathogens, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae (clinical samples), Staphylococcus aureus ATCC43300 (MRSA) and Escherichia coli ATCC25922, in Aurangabad city, India. Four concentrations for each extract (2.5, 5, 7.5, and 10 mg/well) were used. The results of antibacterial activity of methanol extracts at (10 mg/well) concentration of Myrtus communis and Flemingia grahamiana showed maximum zone of inhibition (25.2mm, and 25.7mm) against P. mirabilis and S. aureus, respectively. Extracts of Flemingia grahamiana have not shown inhibition zones against K. pneumonia and E. coli (ATCC 25922). The MIC was tested at a range of concentrations from 25 mg/ml to 0.781 mg/ml for each plant extract. M. communis extracts showed the highest values with MIC of 0.781 for all isolates except E. coli. Also, MIC of methanolic extract of Flemingia grahamiana was 0.781mg/ml for S. aureus and MRSA, and MIC of aqueous extract was 0.781 mg/ml for MRSA.