SYNTHESIS OF PERIWINKLE LEAF AND AMLA FRUIT SILVER NANOPARTICLES AND COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY OF PLANT EXTRACT AND BIOSYNTHESIZED SILVER NANOPARTICLES

The study was performed with Methanolic Periwinkle leaf extract and aqueous Amla fruit extract. The activity was tested on Staphylococcus aureus and Escherichia coli by Agar Well Diffusion Method. Further, Silver Nanoparticles were synthesized from these Plant extract by Biological Method.  Periwinkle leaf and Amla fruit extract showed antimicrobial activity against E. coli and S. aureus and also showed the synthesis of Silver Nanoparticles. The Biosynthesized SNPs were measured on a colorimeter. The λ_max for Periwinkle leaf was found to be 0.32 nm and that 0.65 nm for Amla fruit. This Biosynthesized SNPs of plant extracts was again tested for Antimicrobial Activity using Agar Well Diffusion Method and showed significant inhibition of S. for S. aureus and 24 mm for E. coli. The results of antimicrobial activity for plant extract of Periwinkle leaf and Amla fruit indicates that they inhibit the growth of S. aureus and E. coli. Antimicrobial activity of biosynthesized silver nanoparticles was tested against E. coli and S. aureus; the zone of inhibition obtained by biosynthesized silver nanoparticles of Periwinkle leaf was 37 mm for S. aureus and 42 mm for E. coli. There was no formation of silver nanoparticles of Amla fruit extract using biological method. The results of antimicrobial activity for the biosynthesized silver nanoparticles indicate that Periwinkle leaf inhibits the pathogenic organism S. aureus and E. coli. Hence, the comparative study shows inhibition of S. aureus and E. coli at greater extent.