GC-MS ANALYSIS AND IN-VITRO CYTOTOXICITY STUDY OF DENDROPHTHOE FALCATA AND TRIDAX PROCUMBENS EXTRACTS AGAINST HACAT CELLS LINE

The present study shows the cytotoxic effect of Dendrophthoe falcata (L. f) and Tridax procumbens Linn. extracts against HaCat (Skin cancer) cell line by MTT and SRB assay method. The phytochemical screening of extracts dichloromethane: methanol (DM), methanol: water (MW), and ethanol: water (EW) revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, sterols, and triterpenes. % Cell inhibition by MTT assay of DFDM extract shows 90.00 ± 0.09% and TPDM 91.29 ± 0.02% at 80 µg/ml. IC₅₀ values of DFDM extract <20 µg/ml and TPDM extract 20 µg/ml  compare to IC₅₀ values of standard 5-fluorouracil <10 µg/ml by MTT assay. SRB assay method DFDM extracts shows 86.96 ± 0.07% and TPDM shows 89.14 ± 0.27 with IC₅₀ values < 20 µg/ml compare standard 5-fluorouracil <10µg/ml. HPTLC fingerprint analysis of active DFDM extract showed 11 peaks at 366 nm R_f values 0.06-0.87 & TPDM shows showed 12 peaks  R_f values 0.33- 0.83 at 366 nm. Active extracts were analyzed agilent 7890A GC coupled with triple quadrupole mass detector. GC–MS analysis DFDM extract shows  presence of the 16 phytocompounds, major are [Diethetyl phosphonate- (RT-6.615, 166.075, 43.8.%), Benzyl oxy tridecanoic acid (RT-6.730, MW-320.235, %A-44%), 9(2-phenyl ethyl) Heptadecane (RT-10.233, 344.344,29.1 %), Hexadecanoic acid butyl ester (RT-15.099, 312.302, 65.5%), Linoleic acid, 2,3 bis (O-TMS) propyl esters (RT-23.098,26.3%)]. TPDM extracts shows 19 phytocompounds, major are [Tetradecene (RT-8.249, 198.23, 32.8%), Cyclohexasulfide (RT-9.893, 191.83, 71.4%), Cyclic octaatomic sulphur (RT-15.810, 66.7%), Squalene (RT-22.081, 24.1%). The study showed that the phytochemicals present in the leaves extracts of D. falcata and T. Procumbens were responsible for cell inhibitory activity against the HaCat cell line.