SIMULTANEOUS HPTLC METHOD FOR DETERMINATION OF GALLIC ACID, VANILLIC ACID AND SYRINGIC ACID IN AGRO-INDUSTRIAL WASTE OF DATE FRUITS

Phoenix dactylifera is a medicinally important plant. Fruits are traditionally used for the nutritional purpose. Date seeds are the rich sources of phenolic acids which exhibits strong antioxidant property. HPTLC is a simple, versatile method and it provides visualization of separated constituents. The advantage of this method is this method requires a small amount of sample for analysis. The present work describes a simple, precise, and accurate HPTLC method for quantification of gallic acid, syringic acid, and vanillic acid present in seeds of date fruits. The analysis was done on CAMAG ATS4 automatic TLC sampler system. The chromatographic separation was carried out on precoated silica gel 60 F254 aluminum plates, using a mixture of toluene: ethyl acetate: formic acid (4:4:2 v/v) as mobile phase. The evaluation of spots was carried out at 254 nm using Camag TLC scanner 3. The aimed compounds were resolved satisfactorily with R_f 0.47, 0.53, and 0.60 for gallic acid, syringic acid, and vanillic acid, respectively. The developed method was validated by parameters linearity, accuracy, precision, and repeatability. The correlation coefficients for each marker were greater than 0.99, which meets the validation acceptance criteria. The precision was carried out by intra-day and inter-day precision. Repeatability was measured by scanning spots six times without changing the position of the plate. Phoenix dactylifera seeds are the rich source of phenolic acids, which attribute to antioxidant properties of Phoenix dactylifera seeds. The seeds are the cheaper source that could be useful in medicinal and nutritional areas due to the presence of bioactive phytoconstituents.