PHYTOCHEMICAL ANALYSIS AND PHARMACOLOGICAL EVALUATION OF ETHANOL EXTRACT OF FRUITS OF GREWIA TILIAEFOLIA FOR NEPHROPROTECTIVE ACTIVITY

Aim: The present study was undertaken to screen the phytochemicals present in fruits of Grewia tiliaefolia and to evaluate the potential role of these fruits in the amelioration of cisplatin-induced nephrotoxicity in male Albino rats. Materials and methods: Ethanol extract of Fruits of Grewia tiliaefolia was prepared by hot extraction method. The prepared ethanol extract was subjected to preliminary phytochemical evaluation followed by TLC, HPTLC, HPLC and GC-MS analysis. The nephroprotective potential of the extract was screened at 200 and 400mg/kg b. w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intraperitoneal injection of cisplatin at a dose of 5 mg/kg b.w. The nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels and his to pathological studies in renal tissue. Results: Upon preliminary phytochemical screening, the ethanol extract showed the presence of Proteins, flavonoids, Tannins, Terpenoids. HPTLC analysis resulted in a fingerprint of extract showing the presence of 14 compounds. TLC and HPLC revealed the presence of quercetin, and GC-MS analysis showed the presence of 80 different phytochemicals. Pharmacological studies revealed that administration of extract significantly attenuated the cisplatin- induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in dose-dependent manner. Conclusion: Thus, the findings of the present study provided the phytochemical profile and validated the ethnomedicinal use of fruits of Grewia tiliaefolia as a renoprotective agent.