ANTIOXIDANT, ANTI-ANGIOGENIC AND CYTOTOXIC EFFECT OF ALOE BARBADENSIS, A MEDICINAL PLANT FROM INDIA

Background: Aloe barbadensis (AB) is a member of the Liliaceae family, traditionally used for thousands of years in some parts of India and well known for its anti-inflammatory, antimicrobial, and wound healing properties. The purpose of the study is to evaluate the antioxidant, anti-angiogenic and antiproliferative effect of AB methanolic root extract on A549 lung cancer cell line. Methods: The Antioxidant activity of AB methanolic root extract (ABMRE) was tested using DPPH. The antiproliferative effect against A549-lung cancer cell lines was tested following MTT assay and their anti-migratory activity was tested by performing wound healing or scratch assay. Antiangiogenic property of root extract was determined by measuring the expression of VEGF protein in lung cancer cell by immunoblot technique and in-vivo CAM assay. Results: The ABMRE inhibited DPPH radical up to 50% at the concentration of 60 ± 0.04 µg/ml having high phenol content of 247.50 ± 21.39 mg GAE/g. The cytotoxic effect of ABMRE against A549 cells and their IC₅₀ values were 7.429 ± 0.559, 5.370 ± 0.693, 3.52 ± 0.637 µg/ml for 24, 48, and 72 h respectively. ABMRE inhibited the migration of A549 cells in the wound healing assay was observed. The reduced expression of VEGF protein and inhibition of blood vessel formation in CAM assay by ABMRE was observed. Conclusion: The ABMRE showed antioxidant, antiproliferative, and anti-angiogenic activity could be evaluated further to identify different bioactive compounds having antineoplastic effects as a potential anti-cancer drug for treating lung cancer.