IN-VITRO STUDIES OF EXTRACTS OF PLUMBAGO ZEYLANICA IN CERVIX CARCINOMA CELLS

The present study evaluated the antimicrobial and cytotoxic activities of chloroform, hexane methanol, and petroleum ether extracts of Plumbago zeylanica (PEC, PEH, PEM, and PEP). Antimicrobial activities of the extracts were evaluated against gram-positive bacteria (Staphylococcus aureus-ATCC 6538 and Bacillus cereus-NCIM 2155) and gram-negative bacteria (E. coli-ATCC 8739) along with cytotoxicity activity against cervix carcinoma cell lines (HeLa and SiHa cell lines). Out of the four extracts tested, PEM and PEC extracts showed significantly high value of zone of inhibition against tested bacteria. PEM showed zone of inhibition of 10 mm, 13mm and 8 mm in ATCC 6538, NCIM 2155 and ATCC 8739, respectively. On the other hand, chloroform extract showed a zone of inhibition of 8mm, 10mm and 7 mm in ATCC 6538, NCIM 2155 and ATCC 8739, respectively. The four extracts PEC, PEH, PEM, and PEP showed a significant cytotoxicity activity with IC₅₀ concentration at 238.93ug/ml, 694.44ug/ml, 580.40ug/ml and 593.04ug/ml against the HeLa Cell line and 142.71ug/ml, 547.16ug/ml, 448.04ug/ml and 424.27ug/ml respectively against the SiHa Cell line after the treatment of 24 h. Based on the results of the anti-microbial and anticancer analysis, it was identified that extracts of P. zeylanica may show anticancer activity and also suggest that the PEC has satisfactory cytotoxic potential properties against both cervix carcinoma cell lines based on the dosage of the drug after the incubation period of 24 h. Therefore our results strongly support the antimicrobial and cytotoxicity activities of P. zeylanica provide a scientific basis to develop novel drug formulations from this traditional medicinal plant.