ADVANCEMENT OF GLYCOSIDE HYDROLASE PRODUCTION FROM BACILLUS CEREUS KKSJ 1981 BY STATISTICAL METHOD OF OPTIMIZATION
AbstractPullulanase (Pullulanase-α-glucanohydrolase EC 3.2.1.41) is a glucanohydrolase enzyme that can break down the pullulan and allied oligosaccharides into glucose. Promising of starch-based industries are biofuels, starch syrup, nutraceuticals, etc., increase the glucanohydrolases demand. Present investigation revealing that the Bacillus cereus KKSJ 1981 is enhanced the production of pullulanase through the optimization of temperature, agitation speed, pH, soluble starch concentration, MnSO4 and yeast extract. Response surface methodology (RSM) was utilized to optimize the selected six parameters. A central composite design with 50 experiments was performed in this investigation. The analyzed data depicts that the correlation coefficient (R2) value was 0.9873, and the adjusted R2 value was 0.9717 indicates the high significance of the model. Further, the selected parameters in optimum conditions were predicted through the developed mathematical model, and validation experiments were performed at these conditions. Overall, 1.2 folds of pullulanase were improved through the RSM based optimization.
Article Information
21
1624-1631
1136 KB
379
English
IJPSR
Siva J. Jyothi *, Emandi Hemalatha and Kishore K. Kumar
Hindu college of Pharmacy, Amaravathi Road, Guntur, Andhra Pradesh, India.
jsjbiotech81@gamil.com
24 June 2021
26 July 2021
27 July 2021
10.13040/IJPSR.0975-8232.13(4).1624-31
01 April 2022
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