INVESTIGATION OF ANTI-SWARMING, ANTI-BIOFILM AND ANTI-QUORUM SENSING POTENTIAL OF SOME ORGANIC TEA OF DIFFERENT BRANDS FROM INDIA

The emergency to find a solution to multidrug resistance of bacteria due to the abuse of antibiotics leads to the search for new antibacterial pathways. Quorum sensing (QS), or bacterial cell to cell communication, is a cell-density-dependent bacterial response. It is mediated by hormone-like compounds called autoinducers (AIs). QS-dependent regulation of gene expression controls various phenotypes, including bioluminescence, biofilm formation, drug resistance, virulence factors expression, and motility. Therefore, the inhibition of QS is considered a new promising target of antimicrobial pathways as anti-virulence compounds that can repress the gene expression of QS. Green tea is one of the most commonly consumed teas in the world. Green tea extract is also a great source of compounds like polyphenols, amino acids, enzymes, pigments, and carbohydrates. Hence, green tea has recently received considerable attention as a new source of safe and effective QS inhibitory substances. In the present study, methanolic and ethanolic various tea extracted were prepared and tested to inhibit quorum sensing mediated bacterial virulence factors such as anti-swarming, and antibiofilm potential against organism Pseudomonas aeruginosa. Quorum sensing inhibition against Chromobacterium violaceum MTCC 2656 has been carried out. The study’s outcome shows that most of the methanolic and ethanolic extracts of various tea products show significantly Anti-quorum sensing Potential up to 83% swarming inhibition with Pseudomonas aeruginosa, inhibition violaceum pingment and biofilm disruption were also quite high in most of the Tea extract of ethanol and methanol.