DIVALENT METAL COMPLEX OF COUMARIN: SYNTHESIS & CYTOTOXIC EVALUATION

Introduction: Coumarins are secondary metabolites widely spread in nature. Due to their broad pharmacological activities, many natural and synthetic coumarins and more complex related derivatives are of interest. The coumarin is extremely variable in structure due to the various types of substitutions in their basic structure, influencing their biological activity. Metal complexes of coumarins obtained revealed higher biological activity than their ligand. Metal complexes play a vital role as chemotherapeutic agents. Methods: 7-amino-4-methylcoumarin refluxed with substituted aromatic aldehydes in absolute alcohol in presence of acetic anhydride and was washed with cold water to give Schiff bases of 7-amino-4-methylcoumarin (4a-4j). Further, the metal complexes of coumarin Schiff base (5a-5j) were synthesized by dissolving both Schiff base of 7-amino-4-methylcoumarin and metal sulphates in methanol and are stirred at 250C with dropwise addition of a dil. ammonia. Result and Conclusion: Screening the copper metal complexes against breast cancer cell line (MDA MB 231) with standard Paclitaxel using MTT assay method. The synthesized metal complexes show anticancer activity in a concentration-dependent manner. The IC₅₀ values of metal complexes were found as 258.3, 256.2, 241.3, 262.1, 178.10, 257.8, 175.2, 272.1, 178.3 and 280.6 µg/ml respectively, when compared to standard Paclitaxel IC₅₀ 257 µg/ml under experimental conditions. The primary in-vitro anticancer activity results revealed that 5a, 5b, 5c and 5d showed good anticancer activity.