PRELIMINARY PHYTOCHEMICAL INVESTIGATION, IN-VITRO ANTIOXIDANT, IN-VITRO α-AMYLASE INHIBITION, IN-VITRO AND IN-VIVO ANTICATARACT ACTIVITY OF ETHYLACETATE ROOT EXTRACT OF ABUTILON INDICUM
AbstractThe present study aims to evaluate the in-vitro antioxidant, in-vitro α-amylase inhibition, in-vitro and in-vivo anti-cataract potential of ethyl acetate root extract of Abutilon indicum. Antidiabetic activity was evaluated against alpha-amylase by spectrophotometric assays. DPPH determined antioxidant activity, Reducing power assay, Phosphomolybdenum antioxidant assay, Nitric oxide-scavenging activity, Hydrogen peroxide scavenging, and Hydroxyl radical scavenging assay. Different extract concentrations were made using dimethyl sulfoxide (DMSO) and subjected to an α-amylase inhibitory assay. The in-vitro anticataract potential of Abutilon indicum was determined using glucose-induced cataractous goat eye lens. In the in-vivo group, cataract was induced in rats by 30% galactose diet alone (control) or with the addition of Abutilon indicum (treated group). The results indicate the dose-dependent in-vitro antioxidant activity against DPPH, Reducing power assay, Phosphomolybdenum antioxidant assay, Nitric oxide-scavenging activity, Hydrogen peroxide scavenging, and Hydroxyl radical scavenging comparable with that of standard Ascorbic acid and also appreciable α-amylase inhibitory activity with IC50 values comparable with that of standard Acarbose. An in-vitro study was conducted, which reported that the lens group treated with the plant extract (500μg/ml) exhibited a reduction in opacity compared to the lens in the negative control. The study of the anti-cataract potential of Abutilon indicum exhibited an increase in total protein content, aldose reductase inhibition activity and a decrease in the malondialdehyde level compared to the negative control. Abutilon indicum can delay the onset and progression of cataracts in an experimental rat model of Glucose-induced cataracts in-vitro and galactose-induced cataracts in-vivo.
Article Information
32
827-837
989 KB
329
English
IJPSR
G. V. N. Kiranmayi *, P. Sirisha and R. Sanjana
Faculty of Pharmacy, Dr. M. G. R. Educational and Research Institute, Deemed to be University, Chennai, Tamil Nadu, India.
kiranmayi54@yahoo.com
03 June 2022
01 August 2022
03 August 2022
10.13040/IJPSR.0975-8232.14(2).827-37
01 February 2023