VALIDATED SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF DICLOFENAC DIETYLAMINE
AbstractSimple, sensitive and convenient spectrophotometric methods (method A and method B) are developed for the determination of diclofenac diethylamine (DDEA) in pure form and in formulations. Method A is based on the oxidation of drug by Fe(III) followed by the complex formation between reduced form of Fe(III) with 1,10-phenantroline. The resulting red colored chromogen is measured at 510 nm. Method B is based on the formation of charge transfer complex between DDEA (n-donor) and p-chloroanilic acid (π-acceptor) in 1,4-dioxane. The resulting violet colored chromogen is measured at 526 nm. Beer’s law obeyed in the concentration range of 2.00-8.00 and 20.00-120.00 µg mL-1 for the method A and B, respectively. The molar absorptivity and Sandell’s sensitivity are found to be 0.98 × 104, 1.62 × 103 L mol-1 cm-1 and 3.77 × 10-2, 2.27 × 10-2 µg cm-2 for the method A and B, respectively.The optimum reaction conditions and other analytical parameters are evaluated. Statistical analysis proves that both the methods are reproducible and selective for the determination of DDEA in pure and in dosage form.
Article Information
49
3635-3640
438KB
3638
English
IJPSR
Divya Kumble, Narayana Badiadka* and Sapnakumari Majal
Department of Post Graduate Studies and Research in Chemistry, Mangalore University, Mangalagangothri 574199, Karnataka, India
nbadiadka@yahoo.co.uk
09 May, 2013
03 July, 2013
26 August, 2013
26 August, 2013
01 September, 2013
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