PRODUCTION AND CHARACTERIZATION OF BIOPOLYMER (PHB) USING BACTERIAL STRAINS ISOLATED FROM ALLUVIAL SOIL
AbstractBiopolymers are gaining much interest and are considered appropriate substitutes for petroleum-based plastics. It is used for various applications in the medical, agriculture, and packaging fields. The bacterial strains from river soil samples were isolated and screened for biopolymer production. The objective of the present study was to produce polyhydroxybutyrate (PHB) from the isolated strains under optimized conditions in a shake flask. The optimization was done with the software tool Design expert – Response surface methodology (RSM). The optimum environmental conditions were: initial pH 7.0, Carbon Source 35g/l, and Nitrogen source 1g/l. Under these controlled conditions, biopolymer yield was obtained 2.25g/L. The FT-IR spectra of extracted biopolymer indicate symmetric (O-C-O) presence at 1600cm-1 and asymmetric absorption peak at 1400 cm-1. The TGA result indicates the thermal degradation of the obtained biopolymer was at 106 ℃. The obtained results suggest that these biopolymers may be similar to PHB.
Article Information
27
2347-2353
937 KB
279
English
IJPSR
P. Vishnupriya and S. Ponnarmadha *
Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode, Tamil Nadu, India.
msponnarmadha@gmail.com
27 August 2022
19 October 2022
17 November 2022
10.13040/IJPSR.0975-8232.14(5).2347-53
01 May 2023
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