SECONDARY METABOLITES, IN-VITRO ANTIOXIDANT AND ANTIULCER POTENTIAL OF THE PHYLLOCLADE EXTRACT OF MUEHLENBECKIA PLATYCLADA (F. MUELL) MEISN

The present study aimed to determine secondary metabolites, in-vitro antioxidant, and antiulcer activities using various solvent-based Muehlenbeckia platyclada extracts. Solvents based phylloclade extraction was used to determine various secondary metabolites such as phenols, flavonoids, terpenoids and tannins and to assess in-vitro antioxidant activities such as DPPH, ABTS^.+, superoxide, hydrogen peroxide, nitric oxide, FRAP, Phosphomolybdenum, metal chelating and reducing power activities. Further, in-vitro antiulcer activities such as α-chymotrypsin enzyme, urease enzyme inhibitory activity, H⁺ K⁺ ATPase activity and acid neutralization capacity (ANC) were performed, and the resultant was analyzed using response surface methodology (RSM) to develop an adequate functional relationship between a response of interest (ME) with independent variables (Time, HCL consumed and NaOH consumed). A correlation coefficient was made between secondary metabolites and in–vitro antiulcer activities. Methanolic extract (ME) showed higher levels of secondary metabolites, in–vitro antioxidant activities and was subsequently selected to evaluate its antiulcer potential. A significant inhibition for alpha chymotrypsin (88.84%), urease (68.52%), and H⁺K⁺ ATPase (91.75%) was compared with respective standards. RSM results of ME for ANC indicated that 10 min, 22.66 mL HCl and 30.7 mL NaOH are adequate to get maximum ANC i.e. 46 mEq/ml. A significant correlation (P<0.05) was found between phenol and ACE (R²=0.948), between phenol and HKA (R²=0.941) and phenol and UE (R²=0.952). These results suggest that ME of M. platyclada possesses potential antiulcer activities due to higher levels of secondary metabolites and in–vitro antioxidant activities.