IN-VITRO STUDY OF ANTICONVULSANT ACTIVITY ON THE FRUITS EXTRACTS OF COCCINIA INDICA (WIGHT &ARN.) INDUCED MAXIMAL ELECTROSHOCK SEIZURE (MES) MODEL IN RATS

Aim of the study:In-vitro study of Anticonvulsant activity on the fruits extracts of Coccinia indica (Wight &Arn.) induced maximal electroshock seizure (MES) model in rats.

Materials & methods:  For the assessment of anticonvulsant activity, the animals were divided into five groups of five animals each albino rats. Group I received Normal saline, Group II received Phenytoin, Group III received 200 mg/kg of CIEE suspended in Tween 80, Group IV received 400 mg/kg of CIEE suspended in Tween 80, and Group V received 600 mg/kg of CIEE suspended in Tween 80. The results are expressed in mean ± S.E.M. (n=5) Statistical analysis was done by one way ANOVA, followed Dunnett multiple comparison test vs. control. P<0.01 was considered as statistically significant.

Results: In the present In vitro study, an anticonvulsant effect of ethanolic extracts of fruits Coccinia indica Wight & Arn. has been determined duration of HLTE 14.96, 3.49, 9.68, 8.20, 4.90 sec. The CIEE exhibited maximum anticonvulsant activity at 4.90 sec. at 600mg/kg (P<0.01) and it was significant when compared with control. In the present study, CIEE at the dose of 600mg/kg shows potent anticonvulsant activity as compared to 200mg/kg & 400mg/kg. The ethanolic extract of CI possesses potent anticonvulsant effect against MES induced and it is evidenced by its good anticonvulsant activity of 600 mg/kg against control and as positive control groups.

Conclusions:  In conclusion these observations suggest that 600 mg/kg doses of HLTE exhibit potent anticonvulsant activity.