Posted by admin on Feb 29, 2020 in |
The present study was designed to investigate the cardioprotective effect of Arbutin (ARB) against isoproterenol (ISO) induced myocardial infarction (MI) in rats by studying cardiac and hepatic markers, antioxidant defense system, lipid peroxidation products and histopathological changes. Rats were pretreated with ARB (25 and 50 mg/kg BW) for 21 days. After pretreatment, ISO (60 mg/kg BW) was administered subcutaneously into the rats at an interval of 24 h for 2consecutive days to induce MI. In the present study ISO-induced myocardial damage was indicated by the increased heart weight to body weight ratio and the increased activity of marker enzymes such as CK, CK-MB, ALT, AST, LDH, and the elevated levels of troponin T and I in the serum. In addition, the levels of lipid peroxidation products such as TBARS, CDs, and LHPs were significantly increased in the plasma and heart tissue of ISO rats. Activities of enzymic antioxidants such as SOD, CAT, GPx, GST and the non-enzymic antioxidants like vitamin C, vitamin E and reduced glutathione (GSH) were decreased...
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Posted by admin on Feb 29, 2020 in |
Blood parameters are important indicators for assessing the health status of living organisms subject to changing environment. Blood parameters (Hb%, TEC, TLC, ESR, PCV, MCH, and MCV) of Heteropneustes fossilis exposed to lead were investigated at different time intervals. Acclimatized specimens were divided into four groups: Group-A (Control), Group-B (2.25 ppm), Group-C (2.65 ppm), Group-D (2.85 ppm) of Pb and examined at 7, 14, 21 and 28 days’ time intervals. The maximum impact of the treated metal was observed on ESR (Erythrocyte Sedimentation Rate) [1.80 mm/h (Control) to 2.90 mm/h at 2.85 ppm of lead] during maximum time exposure (28 Days) whereas PCV (Packed Cell Volume) showed a fall [44.00 % (control) to 28.00% (2.85 ppm] at 28 Days pe (post-exposure). The other parameters also showed changes in their values when exposed to lead being highest at 2.85 ppm doses during maximum time exposure (28 Days). The above findings indicate that lead adversely affects the blood physiology as reflected in hematological...
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Posted by admin on Feb 29, 2020 in |
Indian literature relating to the spectrum of infections in maintenance hemodialysis patients has not been extensively published. The purpose of this study was to carry out surveillance of infections in this population and to determine the clinical patterns and outcomes of infections and also to assess the prescribed antibiotic therapy. This was a prospective observational study carried out in the dialysis unit of the nephrology department of a tertiary care teaching hospital. The sample of the study comprised of 30 patients who were receiving maintenance hemodialysis and presented with infections. Escherichia coli was the commonly detected gram-negative pathogen in culture and sensitivity tests. Whereas the most commonly isolated gram-positive bacteria were streptococcus pneumonia and methicillin-resistant staphylococcus species which were sensitive to vancomycin. Respiratory system (47.2%) followed by the urinary system (27.3%) were most commonly affected. The most common outcome of infections was a cure (57%). Clarithro-mycin and ofloxacin-ornidazole were found to be inappropriately dose adjusted. Amoxicillin-clavulanate, cefixime, ticarcillin-clavulanic acid, levofloxacin, piperacillin-tazobactam, mero-penem and imipenem-cilastatin were inappropriately adjusted for dosing...
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Posted by admin on Feb 29, 2020 in |
Endophyte is a rich source of bioactive metabolites for medicinal exploitation as an increasing number of novel compounds with bioactive potential are now being isolated from this source. This study was conducted to characterize and explore endophytic fungi from marine weeds available in Bangladesh. The whole weed was used for isolation of endophytic fungi in the water agar media and the isolated fungi were identified through macroscopic and microscopic observation. Small scale cultivation was performed on liquid Wickersham media. Cytotoxicity, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay, respectively. A total of five endophytic fungi were isolated, purified and characterized from four seaweeds; Hypnea musciformis (HM), Sargassum crassifolium (SC), Dictyota dichotoma (DD) and Caulerpa peltata (CP). They were coded as HMWE-1, HMWE-2, SCWE-1, DDWE-1 and CPWE-1. The fungal strains HMWE-1 and SCWE-1 were identified morphologically as Fusarium sp., HMWE-2 and DDWE-1 as Penicillium sp. and CPWE-1 as Aspergillus sp. DDWE-1 fungal strain showed significant antioxidant activity in comparison...
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Posted by admin on Feb 29, 2020 in |
This study describes the development and validation of stability indicating UPLC method for Bilastine and its impurities, an anti-allergic drug. Bilastine was subjected to stress degradation under different conditions recommended by the International Conference on Harmonization to observe the degradation products. The successful separation of Bilastine from its synthetic impurities and degradation impurities formed under stress conditions were achieved on Acquity UPLC CSH Phenyl-hexyl (2.1 mm × 150 mm, 1.7 µ), and the gradient mode mobile phase consists of 0.05% TFA in water and 0.05% TFA in Acetonitrile. The mobile phase flow rate was 0.10 ml/min. The column temperature was maintained at 25 °C. The sample temperature was maintained at ambient and wavelength fixed at 275 nm UV-detection. It was found that the method of RPUPLC with UV-detection system for the analysis of Bilastine impurities determination and also applied in qualitative and quantitative analysis. The developed UPLC method was validated with respect to specificity, precision, linearity, ruggedness, and robustness. A validation study has been performed as per ICH...
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